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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
01/09/2015 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B.; LOBO JUNIOR, M.; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
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Afiliação: |
MARÍLIA BARROS OLIVEIRA, UFG; MURILLO LOBO JUNIOR, CNPAF; MARIA FATIMA GROSSI DE SA, CENARGEN; SILVANA PETROFEZA, UFG. |
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Título: |
Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Plant Physiology, v. 182, p. 13-22, June 2015. |
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DOI: |
10.1016/j.jplph.2015.04.006 |
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Idioma: |
Inglês |
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Conteúdo: |
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem. MenosSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalan... Mostrar Tudo |
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Palavras-Chave: |
Genes relacionadas com patogénese. |
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Thesagro: |
Doença de planta; Feijão; Fungo; Mofo Branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Thesaurus Nal: |
Dry beans; Methyl jasmonate. |
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Categoria do assunto: |
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H Saúde e Patologia |
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Marc: |
LEADER 02883naa a2200277 a 4500
001 2025021
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jplph.2015.04.006$2DOI
100 1 $aOLIVEIRA, M. B.
245 $aExogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants.$h[electronic resource]
260 $c2015
520 $aSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem.
650 $aDry beans
650 $aMethyl jasmonate
650 $aDoença de planta
650 $aFeijão
650 $aFungo
650 $aMofo Branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aGenes relacionadas com patogénese
700 1 $aLOBO JUNIOR, M.
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tJournal of Plant Physiology$gv. 182, p. 13-22, June 2015.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 13 | |
| 3. |  | BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | OLIVEIRA, C. M.; FREITAS, M. A.; FALEIRO, V. de O.; PETROFEZA, S. Estratégia moleculares para identificação de Meloidogyne incognita, M. javanica, Pratylenchus brachyurus e P. coffese In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 31., 2013, Cuiabá. Anais... Cuiabá, MT: Sociedade Brasileira de Nematologia, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 9. | | MAGALHÃES, L. F.; CUNHA, C. P. R.; LOBO JUNIOR, M.; PETROFEZA, S. Diversidade genética em populações de Sclerotinia sclerotiorum na cultura de feijoeiro (Phaseolus vulgaris L.). Tropical Plant Pathology, Brasília, DF, v. 36, p. 1124, ago. 2011. Suplemento, ref. 1576. Edição dos Resumos do 44 Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 11. | | OLIVEIRA, M. B.; SANTOS, E. M. dos; INOCÊNCIO, A. P. M.; CUNHA, C. P. R.; LOBO JUNIOR, M.; PETROFEZA, S. Análise de enimas hidrolíticas durante a interação do fungo Sclerotinia sclerotiorum e plantas de feijoeiro (Phaseolus vulgaris L.). In: CONGRESSO DE GENÉTICA DO CENTRO-OESTE, 2., 2010, Anais... Goiânia. Goiânia: Universidade Federal de Goiás, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 12. | | BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | BARBOSA, S. R. C.; GOMES, E.; BRESEGHELO, L.; MOURA, A. L. D.; ALVES, E. B.; KNOB, A.; ALMEIDA, A. M. R.; LOBO JUNIOR, M.; PETROFEZA, S. Análise parcial do transcriptoma de Sclerotinia sclerotiorum durante a interação patogênica com feijão comum (Phaseolus vulgaris L.). In: CONGRESSO BRASILEIRO DE GENÉTICA, 52.; CONGRESO DE LA ASOCIACIÓN LATINOAMERICANA DE GENÉTICA, 12., 2006, Foz do Iguaçu. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. 1 CD-ROM. Área Microorganismos - pdf 898.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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